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Glucose Oxidaseis an aerobic dehydrogenase, mainly obtained by fermentation of specific strains of black Aspergillus fungi. The powdered glucose oxidase enzyme is grayish-yellow, liquid glucose oxidase enzyme is light brown, and refined liquid enzyme is pale yellow. It is soluble in water and insoluble in organic solvents such as ether, chloroform, butanol, pyridine, glycerol, and ethylene glycol. 50%acetone and 66%methanol can cause the enzyme to precipitate. When the solution is shaken, the foam is brown-green and cannot pass through nitrocellulose membranes. Solid Glucose Oxidase can be stored for at least 2 years at 0℃. Glucose oxidase is nontoxic and has no side effects on the human body. It is widely used in the food, medicine, and feed industries to remove glucose, deoxidize, and sterilize.
Glucose Oxidase's pH range is between 3.5 and 6.5, with the optimal pH being around 5.0, and the pH range between 4.5 and 7.0 is essentially the same, with little variation. When pH is higher than 7.0 or lower than 4.5, the activity will drop sharply. However, under the same pH conditions, the presence of glucose substrate protects the enzyme activity. For example, at pH 8.1, when there is no glucose substrate, enzyme activity decreases by 90% within 10 min, while in the presence of glucose substrate, enzyme activity only decreases by 20% within 40min. Under low pH conditions, mold glucose oxidase enzyme still has certain catalytic activity, but the reaction rate is lower, and special catalytic reactions can still be completed.
Glucose Oxidase's working temperature range is generally between 30 and 60℃, and the optimal working temperature is between 50 and 55 ℃. The reaction system of glucose oxidase enzyme contains gaseous reactant oxygen. Therefore, changes in reaction temperature lead to changes in the concentration of oxygen in the reaction system. When the temperature increases, the solubility of oxygen in the reaction system decreases, which offsets the effect of temperature on the reaction rate of the enzyme.
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