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Keratinase

Protease catalyzes peptide bond and hydrolyzes protein into peptone, hydrazone, polypeptide and amino acid.  As a kind of alkaline serine protease, keratinase not only has the function of normal alkaline protease, but also can break down disulfide bond and disintegrate keratin.

Bestzyme Keratinase
Bestzyme Keratinase
Bestzyme Keratinase
Bestzyme Keratinase


Specification of Keratinase


Name

Keratinase

Product Series Name

NutriGen Keratinase

Activity

100000-200000 U/g

Strain

Parengyodontium album

EC code

EC 3.4.99.11

Form

Powder,Liquid

Carrier

Starch

Color

White, Light yellow

Loss on drying

≤ 10%

Working pH

6.0-12.5

Optimum pH

11

Working T

30℃-70℃

Optimum T

60℃

Particle size

80% pass 40 mesh

Package

25kg /bag

Storage

Stored 6 months in dark, cool and dry conditions

Caution

Avoid direct sunlight, high temperature



Definition and Dosage of Activity Keratinase

One unit (U) of protease activity is defined as the amount of enzyme which liberates 1 μg tyrosine per minute from substrate casein at 40℃ and pH 10.5 under the conditions of the test.

Recommended dosage is 50-100g/t with 50000 U/g in complete feed.


Functions of Keratinase

1. Supplement the deficiency of endogenous protease for animal.

2. Increase the digestibility of protein and amino acid in poultry and swine feed ingredients.

3. Degrade the trysin inhibitor and lectin, this is what endogenous protease can’t do.

4. Optimized the use of lower digestibility feed ingredient e.g corn, sorghum, feather meal,soybean meal and blood plasma powder.

5. Creat a better environment for animal and decrease pollution from nitrogen for human.


Application of Keratinase

1. The matrix value of keratinase (100g/t)

Nutrient

Poultry

Value

Amount provided in the diet

Metabolizable energy(Mcal/kg)

600

0.060

Crude protein, %

5400

0.540

Lysine, %

150

0.015

Methionine, %

180

0.018

Cystine, %

150

0.015

Threonine, %

210

0.021

Tryptophane, %

90

0.009

Isoleucine , %

150

0.015

Arginine, %

285

0.0285

Valine, %

255

0.0255


2. Application case: Improve the performance and digestibility of nutrients.

Experimental animal: 840 one-day-old AA broilers, 6×35 broilers/treatment.

Experimental design:

Table1. Experimental design

Item

Diet

PC

Basal diet

NC

Basal diet-0.5% crude protein

Group I

NC+125g/t competitor

Group II

NC+100g/t Keratinase(30000U/g)

Experimental cycle: 42 days

Results:

Table2  Effect of each treatment on the growth performance of AA broilers (g, %)

Items

PC

NC

Group I

Group II

Initial weight,g

62.79±0.31

62.58±0.29

62.56±0.33

62.33±0.27

1-21days

21stAG,g

986.16ab±10.08

977.41b±8.22

1013.59ab±25.72

997.17ab±10.53

ADFI,g

62.08± 1.43

60.93±1.10

62.09±2.04

58.71±2.12

ADG,g

43.94±0.47

43.45±0.39

45.12±1.29

44.33±0.53

F/G

1.41±0.03

1.40±0.03

1.38±0.05

1.33±0.04

Death rate,%

1.43±1.57

3.45±2.21

5.38±6.24

3.33±5.24

22-42days

22ndAG,g

1140.08±1.30

1135.33±5.97

1136.73±7.06

1138.33±5.41

42ndAG,g

2985.77±41.56

2971.06±47.61

3058.21±58.29

3010.25±44.17

ADFI,g

179.00±3.12

178.64±2.86

175.81±3.64

174.22±2.64

ADG,g

87.88±2.01

87.42±2.43

91.50±2.85

89.14±2.20

F/G

2.04±0.07

2.05±0.03

1.93±0.05

1.96±0.06

Death rate,%

2.00±2.19

1.33±2.07

0.67±1.63

1.33±2.07

1-42days

ADFI,g

120.51±2.39

119.78±1.63

118.94±2.01

116.47±1.60

ADG,g

69.59±0.98

69.25±1.13

71.32±1.39

70.18±1.05

F/G

1.73±0.02

1.73±0.02

1.67±0.02

1.66±0.03

Death rate,%

3.43±3.76

4.78±1.73

6.05±5.74

4.67±4.59

Note: In the same row, values with different small letter superscripts mean significant difference(P<0.05).


Diagram1 Effect of keratinase on the nutrient digestibility of broilers

Keratinase


Table3 Effect of keratinase on the amino acid digestibility of broilers (%)

Items

PC

NC

Group I

Group II

Asp

55.87±4.44

52.59±2.57

54.52±8.03

57.52±5.76

Thr

63.09±7.47

61.47±5.32

62.17±5.74

63.69±5.19

Ser

69.11±5.51

65.44±4.61

65.80±7.04

68.04±4.10

Glu

69.31±3.41ab

64.68±1.35b

67.12±5.82

74.82±3.39a

Gly

55.36±15.14ab

42.03±4.21b

49.11±11.59b

67.00±1.89a

Ala

31.82±2.11b

34.51±4.37b

35.35±5.19

35.92±4.82b

Val

46.32±0.75

45.59±1.66

44.68±4.68

50.18±3.73

Met

72.53±1.35

70.69±2.65

75.17±3.79

74.29±3.31

Ile

46.45±0.53b

47.40±2.87ab

47.47±5.60

51.65±4.65ab

Leu

56.28±1.57

52.87±7.19

55.44±8.01

53.05±4.90

Tyr

50.93±3.67

47.91±4.34

50.94±10.97

56.55±8.06

Phe

66.11±2.37

61.58±1.25

62.81±7.79

68.80±6.93

His

31.90±4.72ab

25.09±7.33b

28.06±14.13

40.79±5.43ab

Lys

78.58±4.31ab

71.44±2.54b

74.47±8.35

82.45±1.20a

Arg

79.15±2.86ab

75.18±3.10b

75.80±5.99

80.68±3.36ab

Pro

67.76±0.73b

70.57±1.32ab

69.36±7.39

68.06±2.90b

TAA

62.69±0.43ab

59.17±1.39b

60.83±5.69

66.45±3.73a


Conclusion of Keratinase

On the basis of these results, it is concluded that the supplement of keratinase into a low CP diet was as effective as normal level in broiler. As shown in diagram 1, keratinase improve the digestibility of dry matter, crude protein, crude fat, general energy and apparent metabolic energy. And in table 4, karatinase has a positive effect on the amino acids digestibility. In this experiment, it is appeared that karatinase improve the growth performance of broiler by increase average diary gain and feed conversion rate.




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