Catalase is a new and functional enzyme preparation. Catalase is widely distributed in the biological world, almost from animals to plants, and even from humans to single-celled organisms. Catalase is the natural enemy of oxygen free radicals (reactive oxygen species, ROS) and mycotoxins (oxidizing cell membranes, proteins and nucleic acids). It is the number one killer of oxygen free radicals in the body and the foundation of life and health. It is an active substances derived from living organisms that can eliminate harmful substances produced in the metabolic process of living organisms. And it is regarded as the most magical enzyme in life science and the garbage scavenger in the human body. Continuously supplementing catalase to the body has the special effects of detoxification, disease resistance, liver and kidney protection, protecting and repairing of gastrointestinal mucosa, increase of feed intake, and prevention of cell aging.
Peroxisomes contain more than 40 kinds of enzymes. The types and proportions of oxidases contained in each peroxisome are different, but catalase enzyme is present in the peroxisomes of all cells, accounting for approximately 40% of the total amount of peroxisome enzymes, so catalase is the marker enzyme of peroxisome. The functions of various peroxisomes are different, but the functions of oxidizing a variety of substrates, catalyzing the generation and decomposition of hydrogen peroxide are common. In the process of oxidizing the substrates, oxidase can reduce oxygen to become Peroxide, and catalase enzyme can reduce hydrogen peroxide to water, so the main function of peroxisomes in liver and kidney cells is to prevent excessive hydrogen peroxide from being produced, so as to prevent cell poisoning and protect cells.
Catalase enzyme is a powder with color of white to light brown, and its molecular weight is about 240KDa, isoelectric point is 5.2, α-helical structure. It is soluble in water, but almost insoluble in organic solvents such as ethanol, chloroform and ether. The enzyme is ferroporinase, which contains 4 iron atoms in a molecule, and its functional group is heme.
Action temperature: 33-40℃
Action PH: 6.5-7.5
Ca2+ has a protective and activating effect on it, and fluorine can induce a significant increase in catalase activity. Cyanide and azide cyanide can bind to heme and are reversible inhibition.
Surface active compounds can significantly inactivate catalase, and radiation can significantly reduce the thermal stability of the enzyme.
Catalase is tested at different pH values, different temperatures, and half of the inactivation time.
Unit: second, half inactivation.
Standard: 50 IU/g
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